Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na^+-ATPase

Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na^+-ATPase  

 

Taeho Jo, Mariko Shono, Masato Wada, Sayaka Ito, Junko Nomoto, and Yukichi Hara

Membrane 35(2), 80-85, 2010-03-01 

日本膜学会

 

[Abstract]

The three–dimensional structure of Heterosigma akashiwoNa+–ATPase (HANA) was predicted by means of homology modelingbased on the crystal structure of the K+–bound form of shark Na+/K+–ATPase (PDB ID: 2ZXE). The overall structure of HANA appearsto be similar to that of shark Na+/K+–ATPase.Both contain three characteristic cytoplasmic domains, A, N and P, which are uniqueto P–type ATPases.HANA has a long TM7–8 junction as a large extracellular domain, in place of theβ–subunit of shark Na+/K+–ATPase.Two putative K+–binding sites in the transmembranedomain of HANA were identified by means of valence mappingbased on the constructed structure. The presence of K+–bindingsites and the reported ion requirements for ATPase activity and EPformation indicate thatHANA may transport K+ ions in the samemanner as animal Na+/K+–ATPases.

 

Key words:Heterosigma akashiwo／marine alga／Na+/K+–ATPase／homology modeling／K+–binding sites 

Citation information

MLA	Jo, Taeho, et al. "Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na^+-ATPase." Membrane 35.2 (2010): 80-85.
APA	Jo, T., Shono, M., Wada, M., ITO, S., NOMOTO, J., & HARA, Y. (2010). Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na^+-ATPase. Membrane, 35(2), 80-85.
Chicago	Jo, Taeho, Mariko Shono, Masato Wada, Sayaka ITO, Junko NOMOTO, and Yukichi HARA. "Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na^+-ATPase." Membrane 35, no. 2 (2010): 80-85.

 

CiNII

http://ci.nii.ac.jp/naid/10026877117/

 

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